Lasp-1 recruitment is associated with early stages of podosome biogenesis.

<p>(<b>A</b>) Colocalization of recombinant EGFP-Lasp-1 and recombinant mRFP-actin at podosomes in PDBu-treated A7r5 cells. The white box marks the enlargement shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035340#pone-0035340-g003" target="_blank">Fig. 3B</a>. Bar represents 25 µm. (<b>B</b>) Appearance of EGFP-Lasp-1 during assembly and disassembly of podosomes (white boxes) in PDBu-treated cells over a period of 4 min. Inserts in the pictures taken at 0 s, 2 min. and 4 min. show the merged EGFP-Lasp-1 (green) and mRFP-actin (red) signals in two individual podosomes. Bar represents 2 µm. (<b>C</b>) Colocalization of EGFP-Lasp-1 (upper row) and DsRed-cortactin (middle row, merged pictures in the lower row) during initial stages of podosome assembly in PDBu-treated A7r5 cells over a period of 2 min. An individual podosome is marked by a red circle. Bars represent 25 µm (left panel) or 2 µm (right panels), respectively.