LAMP-2 decorates the neutral-lipid-rich vesicles in macrophages incubated with Chs-LDL.

<p>Macrophages incubated for 48 h with Ac-LDL (A-<b>A^IV</b>) or Chs-LDL (B-<b>B^IV</b>) were fixed and double labelled with anti-LAMP-2 antibodies and Bodipy. Merged images (panels (A-<b>A^I</b>) and (B-<b>B^I</b>)) show LAMP-2 in red and Bodipy in green. Panels (A<b>^I</b>) and (B<b>^I</b>) are the zoomed regions outlined by the rectangles in panels (A) and (B), respectively. Panels (A<b>^II</b>) and (B<b>^II</b>) show LAMP-2 staining. Panels (A<b>^III</b>) and (B<b>^III</b>) show lipid droplet staining. All panels show confocal single slice images. Asterisks point to LAMP-2-positive lipid droplets. Bars, 10 µm. The graphs (A<b>^IV</b>) and (B<b>^IV</b>) show relative intensity scans along the arrows in panels (A<b>^I</b>) and (B<b>^I</b>), respectively, of the LAMP-2 (red line) and Bodipy (green line) stains.