Cell specificity of AAV2/8 transduction.

<p>(<b>a</b>–<b>d</b>) Anti-CD34 staining (in red) of a non-injected mouse cornea shows abundant CD34+ cells. (<b>e</b>–<b>h</b>) Anti-CD34 labelling (in red) of a mouse cornea 24 h post-injection with 3×10⁹ vg of AAV2/8. Intra-stromal injection results in a decrease in the proportion of CD34+ cells (in red) due to an augmentation in the number of cell nuclei (in blue). The EGFP signal co-localises (arrows and arrowheads) with the CD34-labelled cells. Inset in <b>h</b> shows a higher magnification of the cells indicated by arrowheads minus the Hoechst filter. (<b>i</b>–<b>l</b>) Anti-F4/80 staining (in red) of a non-injected mouse cornea showing a low number of F4/80+ cells. (<b>m</b>–<b>p</b>) Anti-F4/80 labelling (in red) of a mouse cornea 24 h post-injection with 3×10⁹ vg of AAV2/8. The EGFP signal does not co-localise (arrows) with the F4/80-labelled cells.