Subcellular distribution of AGT variants.

<p>(A) Human fibroblast cells were transfected with plasmids expressing EGFP-fused AGT variants, which are labeled. Congruent punctuate fluorescence pattern of wt-EGFP-AGT and Pex14p demonstrates peroxisomal localization. Additional diffuse cytosolic staining obtained for all mutants indicates partial cytosolic mislocalization. Large fluorescent patches that are not congruent with the characteristic peroxisomal pattern are the result of protein aggregation. Size bars, 10 µm. Additional images are shown in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001309#pbio.1001309.s007" target="_blank">Figure S7</a>. (B) Histogram showing the percentages of transfected cells displaying distinct EGFP-AGT localization patterns (green, peroxisomal; blue, cytosolic; red, aggregates). Standard deviations are shown with error bars.