p53 family-mediated stimulation or degradation of cutaneous HPVE6 is HPV type dependent.

<p>Expression of HPVE6 genes in H1299 cells (p53-null) with co-expression of wtp53, mutp53R248W or ΔNp63α (1 µg each). HPV types included (A) HPV4E6, 48E6 and 60E6 of genus Gamma-papillomavirus, (B) HPV5E6, 20E6 and 38E6 from genus Beta-papillomavirus, (C) HPV7E6, 27E6 and 77E6 from genus Alpha-papillomavirus and (D) HPV41 from genus Nu-papillomavirus. Proteins were analysed by Western blot analyses and levels were quantified by Imagequant. Histograms represent HPV E6 protein expression normalized against β-actin loading control. Empty vector pcDNA3.1 (+) was co-transfected to equalize the total amount of transfected DNA. Transfection efficiency was controlled for by β-galactosidase expression. (E) RT-PCR <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035540#pone.0035540-Fei2" target="_blank">[40]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035540#pone.0035540-Fei3" target="_blank">[59]</a> demonstrating the respective mRNA of c-terminal HA-tagged HPVE6 genes.