SAA pro-inflammatory effects in hMADS adipocytes are not due to endotoxin contamination.

<p>Fully differentiated hMADS adipocytes were preincubated with or without 2 µM TAK-242 inhibitor for 1 h. Upon preincubation, cells were treated in the presence or absence of recombinant human SAA (1, 3 and 10 µg/mL) or LPS (1, 3 and 10 ng/mL) for 24 h. At the end of the treatment period, the supernatants were recovered and secreted MCP-1 (Panels A and B) and IL-6 (Panels C and D) were measured by ELISA. Data are expressed as mean ± SD from 3 independent experiments.