HPV-16 entry requires actin dynamics but not Rho-GTPase or Arf6 signaling.

<p>HeLa cells were pretreated for 30 min (A, B, G) or overnight (C) with cytochalasin D (A), jasplakinolide (B), C3 toxin/toxin B (C), and EIPA (G) in the indicated concentrations. Cells were infected with HPV-16 PsV (black), SFV (grey), or SV40 (white) and analyzed as in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002657#ppat-1002657-g002" target="_blank">Figure 2C</a>. Alternatively, HeLa cells were transfected with Cdc42, Rac1, RhoA (D, E), or Arf6 (F) fused to mRFP. Either the wildtype (WT, black), the DN (grey), or the CA (white) mutant were used. 24 h post transfection cells were infected with HPV-16 PsV (D) or VV (E). Infection was scored by flow cytometry as in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002657#ppat-1002657-g003" target="_blank">Figure 3A–C</a>. Depicted are results normalized to mRFP expressing control cells.