Signaling events required during HPV-16 entry.

<p>(A–H) HeLa cells were pretreated for 30 min with genistein (A), H-7 (B), okadaic acid or orthovanadate (C), iressa (D), calphostin C (E), wortmannin (F), PI-103 (G), or IPA-3 (H) in the indicated concentrations. (A–C, E–G) Cells were infected with HPV-16 PsV (black), SFV (grey), or SV40 (white) and analyzed as in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002657#ppat-1002657-g002" target="_blank">Figure 2C</a>. (D) Cells were infected with HPV-16 PsV (black), SFV (grey), or VV (white). Infection was scored by automated microscopy and image analysis as in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002657#ppat-1002657-g002" target="_blank">Figure 2C</a> (HPV-16, VV) or by flow cytometry (SFV). (H) Cells were infected with HPV-16 PsV (black), SV40 (grey) or VV (white). Infection was scored by automated microscopy and image analysis as in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002657#ppat-1002657-g002" target="_blank">Figure 2C</a> (HPV-16, VV) or by flow cytometry (SV40). (I) HeLa cells were transfected with siRNA oligos directed against PAK-1 or with the AllStar Negative control (Qiagen). 48 h after siRNA transfection cells were infected with HPV-16 PsV (black), VSV (grey), and VV (white). Infection was scored as in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002657#ppat-1002657-g002" target="_blank">Figure 2C</a> and depicted relative to cells transfected with the AllStar negative control.