Sequences flanking the CRG elements from the AD genome hybridize to the centromeres of the non-CRG-containing <i>Gossypium</i> genomes.

<p>DAPI-stained cotton interphase or mitotic nuclei (blue) from <i>Gossypium</i> species, as indicated, were hybridized with a CRG-containing BAC, GH60L12 (red), which shows localization to the centromere region in the tested <i>Gossypium</i> species, including those species that do not contain the CRG element in their genomes (A, E1, and F1). Interphase spreads (top panels) show strong foci of the correct number, consistent with centromere localization. Also, metaphase chromosomes (bottom panels) show localization to the primary constriction.