HENN-1 Is Broadly Expressed in <i>C. elegans</i> Germline.

<p>A) The <i>henn-1</i> mRNA expression profile is consistent with germline enrichment. Levels of <i>henn-1</i> mRNA were assayed throughout development and normalized to <i>eft-2</i> mRNA. Standard deviation is shown for biological triplicates. Non-normalized levels are shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002617#pgen.1002617.s013" target="_blank">Figure S13A</a>. B) HENN-1 is detected at all stages of development and in male. Lysates from animals of the indicated stages were probed with anti-HENN-1 rabbit polyclonal antibody. C) HENN-1 is abundant in hermaphrodite proximal germline and enriched in proximal oocyte nucleoplasm (inset). Extruded gonads of <i>xkSi1; henn-1(tm4477)</i> adult hermaphrodites were stained with anti-GFP mouse monoclonal and anti-HENN-1 rabbit polyclonal antibodies. D) HENN-1 is detectable in male proximal and distal gonad, with enrichment in residual bodies during spermatid maturation (inset). Extruded gonads of <i>xkSi1; henn-1(tm4477)</i> adult males were stained with anti-GFP and anti-HENN-1 antibodies. E) Expression of endogenous HENN-1 mirrors expression of HENN-1::GFP from transgene <i>xkSi1</i>. Extruded gonads of wild-type animals were stained with anti-HENN-1 antibody. F) Detection of HENN-1 proteins by immunostaining is specific. Extruded gonads of <i>henn-1(tm4477)</i> mutant animals were stained with anti-GFP and anti-HENN-1 antibodies. E, embryo.