Pex16 is not essential for peroxisome biogenesis.

<p>Fluorescence microscopy analysis of <i>P. chrysogenum</i> Δ<i>pex16</i> GFP.SKL (<b>A</b>) and Δ<i>pex11</i> Δ<i>pex11B</i> Δ<i>pex11C</i> Δ<i>pex16</i> GFP.SKL (<b>B</b>) cells. Cells were grown for 40 h in PPM. For corresponding WT see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035490#pone-0035490-g003" target="_blank">Fig. 3A</a>. Scale bars represent 5 µm. <b>C</b>. Cells devoid of <i>pex16</i> are sporulation deficient. Colonies of WT and both Δ<i>pex16</i> GFP.SKL and Δ<i>pex11</i> Δ<i>pex11B</i> Δ<i>pex11C</i> Δ<i>pex16</i> GFP.SKL strains were grown for 7 days on sporulation inducing R-agar plates. <b>D</b>. Δ<i>pex16</i> cells are characterized by decreased levels of Pex11. Western blots of WT and Δ<i>pex16</i> GFP.SKL cell extracts were prepared and decorated with α-Pex11 antibodies; translation elongation factor 1- (eEF1A) was used as a loading control. <b>E</b>. <i>P. chrysogenum</i> cells lacking <i>pex16</i> are able to grow on oleic acid although at decreased rates. WT, Δ<i>pex16</i> GFP.SKL and Δ<i>pex11</i> Δ<i>pex11B</i> Δ<i>pex11C</i> Δ<i>pex16</i> GFP.SKL strains were grown for 10 days on mineral medium containing 0.5% glucose or 0.1% oleic acid as a sole carbon source. Electron micrographs of Δ<i>pex16</i> GFP.SKL (<b>F</b>) and Δ<i>pex11</i> Δ<i>pex11B</i> Δ<i>pex11C</i> Δ<i>pex16</i> GFP.SKL (<b>G</b>) cells grown for 40 h in PPM; P – peroxisome; M – mitochondrion; V – vacuole; arrows indicate protein dense inclusions. Scale bars represent 1 µm. Electron micrographs representing α-IAT immunolabelling of sections of Δ<i>pex16</i> GFP.SKL (<b>H</b>) and Δ<i>pex11</i> Δ<i>pex11B</i> Δ<i>pex11C</i> Δ<i>pex16</i> GFP.SKL (<b>I</b>) cells. Scale bars represent 1 µm.