Morphology of wild-type and defective D-type motor neuron commissures.

<p>(A) Full body image of adult worm. Scale bar is 25 µm. (B) Expanded view of area within dashed box in panel A. Ventral nerve cord (VNC) is distinguished by presence of neuronal cell bodies. Dorsal nerve cord labeled DNC. Scale bar is 25 µm. (C) Representative image of panel B. Black arrows label neuronal commissures while black arrowheads identify neuronal cell bodies. Respective phenotypes characterized by: (D) commissure never reaching dorsal nerve cord; (E) commissure running along the opposite side of the animal's body (left, colored yellow); (F) absent commissure; (G) additional commissure present; (H) two commissures crossing over each other; (I) two commissures entering the dorsal nerve cord or leaving the ventral nerve cord together, they may also partially fasciculate; (J) bifurcating commissure; (K) neighboring commissures joined by a process; (L) neurite with length less than half nematode width; (M) an absence of GFP expression along either dorsal or ventral nerve cords; (N) break in GFP expression in a commissure. Scale bar is 25 µm. Representative image of phenotype shown beneath each photo accompanied with illustrative phenotype symbol. (O) Proportion of animals in a population with at least one incidence of each independent defect. Error bars represent standard error of proportion.