miR-133b induces increased apoptosis in DR receptor stimulated PC3 cells.

<p>Cells were transfected with miR-133b (red) or a control miR (ctrl miR, black), as reference. After 48 h, cells were either left untreated (Unstim) or stimulated for 4 h with 20 ng/ml tumor necrosis factor-alpha (TNFα), 100 ng/ml of a cross-linking activating antiFas antibody (αFas/CD95) or 20 ng/ml recombinant human TRAIL (rhTRAIL). Treated cells were harvested, stained and scanned by flow cytometry for the presence of cleaved active caspase 8 (left) and caspase 3 (right). 7-Amino-actinomycin D (7-AAD) was used for exclusion of cells with compromised membrane integrity from the caspase activation quantification assay.