VCP positively regulated the progress of HCC.

<p>A: Analysis of the level of VCP in HCC tissues by qRT-PCR. The result showed that the level of VCP is frequently increased in human HCC tissues. *:P<0.05.**:P<0.01. B,C: Effect of si-VCP and miR-129-5p on tumor growth in nude mice model. si-VCP, miR-129-5p and NC-transfected HepG2 cells were suspended and then injected subcutaneously into either side of the posterior flank of the male BALB/c athymic nude mouse. Tumor growth was examined every four days. Tumor volume (V) was monitored by measuring the length (L) and width (W) of the tumor with calipers and was calculated with the formula V = (L×W²)×0.5. Photographs of dissected tumors from nude mice (B) and the curve of tumor growth (C) are shown. *:P<0.05. Results are representative of three animals in every time point per group. D:The level of VCP and Bcl-2 in the transplanted tumors was detected by IHC.