Motility of cells overexpressing SH2B1β is higher during healing days 0–4 and reduced during healing days 5–8.

<p>PC12-GFP and PC12-SH2B1β cells were differentiated and subjected to wound healing as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034999#pone-0034999-g001" target="_blank">Figure 1</a>. (A) Cell migration was tracked every day and the net distance per day was calculated. Values are mean ± S.E.M. from four independent experiments for healing days 0–6 (left panel) and three independent experiments for healing days 0–1 and 7–8 (right panel). (*: P<0.05, paired Student's t-test) (B) Relative pERK1/2 levels were normalized to total ERK1/2 and then the levels in PC12-GFP cells on differentiated day 8 (Un-wounded) or on healing day 7. Values are mean ± S.E.M. from eighteen independent experiments for un-wounded cells and four independent experiments for healing day 7. (*: P<0.05, paired Student's t-test) (C) SH2B1β modulates PKC-dependent cell migration and ERK1/2-mediated neurite re-growth depending on the healing stages. PKC-dependent cell migration antagonizes ERK1/2-mediated neurite re-growth, and fine temporal regulation of cell migration and neurite re-growth contributes to the optimal healing outcome.