Hoxc8 is down-regulated during brown adipogenesis in vivo.

<p>(A) Immunofluorescence analysis of Hoxc8 in the mouse SVF cells derived from inguinal WAT. The cells were untreated (Undifferentiated) or induced to undergo differentiation (Adipogenic induction). The lipid droplets and nuclei were counterstained with Bodipy and DAPI, respectively. Scale bars indicate 30 µm. (B) Immunoblots of Hoxc8 in the mouse SVF cells left untreated or induced to undergo differentiation. β-actin served as a loading control. (C) Upper, the UCP1 expression in the differentiated mouse SVF cells. Scale bars indicate 30 µm. Lower, the fold increase of mRNA expression levels of <i>Ucp1</i> and <i>Ucp2</i> in the mouse WAT-SVF cells induced to undergo differentiation. The results were normalized to <i>β-actin</i>. (D) The expression of <i>Pgc-1α</i> and <i>C/EBPβ</i> induced during the differentiation of mouse SVF cells. The results were normalized to <i>β-actin</i>. The data are presented as means ± SEM; * <i>p</i><0.05. (E) Western blot analysis in different fat depots from mice treated with or without CL-316,243, a β3-adrenergic receptor agonist. ingWAT, epiWAT, and iBAT denote inguinal WAT, epididymal WAT, and interscapular BAT, respectively. (F) Western blot analysis of Hoxc8 in SVF cells and ingWAT of mice treated with CL-316,243 (CL) or saline.