Characterization of hiPSC generated either from fibroblasts or CB-CD34+ HSPCs.

<p>All the hiPSC lines generated from either fibroblasts or CB-CD34+ HSPCs express the pluripotency markers Oct4, Sox2, Nanog and Rex1 (<b>A</b>), the pluripotent surface markers Tra-1-60, Tra-1-81, SSEA-3 and SSEA-4, as well as nuclear Oct4 (<b>B</b>) and display an euploid karyotype (<b>C</b>). All hiPSC lines differentiated <i>in vivo</i> (teratoma formation) (<b>D</b>) into tissues representing the three germ layers: ecto-, meso- and endoderm. Immunocytochemistry pictures are shown at 20× magnification. (<b>E</b>) RT-PCR confirming that CB-iPSC#2 and F-iPSC#2 did not silence OKSM factors after >20 passages.