<p>PBMC isolated from 40 healthy BCG-vaccinated individuals were stimulated with Mtb cell wall antigens (CW) or peptide pools derived from Mtb-specific proteins ESAT-6 and CFP-10 (10 mg/ml). Data are represented as spot forming cells (SFC) per 2×10<sup>5</sup> PBMC. Each data point corresponds to a single donor.</p
<p>IFN-γ ELISPOT assay was performed to evaluate T-cell responses against the 27 M-group consensus p...
<p>IFN-γ production was measured in culture supernatants of HLA-A2<sup>+</sup> recovered individuals...
<p>A) BEAS-2B cells were seeded in a 12-well tissue culture plate and a 0.4 uM pore size transwell i...
<p>CD8<sup>+</sup> T cells from four healthy, eight LTBI, and four Mtb-infected donors were incubate...
<p>PBMCs were initially screened by <i>ex vivo</i> ELISpot and the CD4<sup>+</sup> and CD8<sup>+</su...
<p>(<b>A</b>) BALB/c, (<b>B</b>) C57BL/6 and (<b>C</b>) C3H/HeN mice were immunized once with either...
Activated CD3+CD4+ and CD3+CD8+ T cell populations of PBMCs from BCG+ donors (solid bars) and BCG- d...
a<p>Spot forming cell numbers represent an individual donor (in bold) and entries are the means of s...
<p>Responses were measured by stimulating whole blood for 6 days and assaying the culture supernatan...
ELISPOT, ELISA and flow cytometry techniques are often used to study the function of immune system c...
<p>(<b>A</b>) Overview of primate experimental design. Three primates were immunized thrice with MVA...
<p>(A,B) Human monocyte-derived DC were treated with epoxomicin, BFA, or bafilomycin for one hour be...
<p>A–B) Mtb-infected DC, BEAS-2B cells, or LAEC (MOI:10) were used as APCs in an IFN-γ ELISPOT assay...
<p>IFNγ ELISpot responses specific to Mtb antigens following injection of MVATG18598 in <b>(A)</b> B...
<p>Analyses of IFNγ in the culture supernatant by ELISA after 7 days and two rounds of <i>in vitro</...
<p>IFN-γ ELISPOT assay was performed to evaluate T-cell responses against the 27 M-group consensus p...
<p>IFN-γ production was measured in culture supernatants of HLA-A2<sup>+</sup> recovered individuals...
<p>A) BEAS-2B cells were seeded in a 12-well tissue culture plate and a 0.4 uM pore size transwell i...
<p>CD8<sup>+</sup> T cells from four healthy, eight LTBI, and four Mtb-infected donors were incubate...
<p>PBMCs were initially screened by <i>ex vivo</i> ELISpot and the CD4<sup>+</sup> and CD8<sup>+</su...
<p>(<b>A</b>) BALB/c, (<b>B</b>) C57BL/6 and (<b>C</b>) C3H/HeN mice were immunized once with either...
Activated CD3+CD4+ and CD3+CD8+ T cell populations of PBMCs from BCG+ donors (solid bars) and BCG- d...
a<p>Spot forming cell numbers represent an individual donor (in bold) and entries are the means of s...
<p>Responses were measured by stimulating whole blood for 6 days and assaying the culture supernatan...
ELISPOT, ELISA and flow cytometry techniques are often used to study the function of immune system c...
<p>(<b>A</b>) Overview of primate experimental design. Three primates were immunized thrice with MVA...
<p>(A,B) Human monocyte-derived DC were treated with epoxomicin, BFA, or bafilomycin for one hour be...
<p>A–B) Mtb-infected DC, BEAS-2B cells, or LAEC (MOI:10) were used as APCs in an IFN-γ ELISPOT assay...
<p>IFNγ ELISpot responses specific to Mtb antigens following injection of MVATG18598 in <b>(A)</b> B...
<p>Analyses of IFNγ in the culture supernatant by ELISA after 7 days and two rounds of <i>in vitro</...
<p>IFN-γ ELISPOT assay was performed to evaluate T-cell responses against the 27 M-group consensus p...
<p>IFN-γ production was measured in culture supernatants of HLA-A2<sup>+</sup> recovered individuals...
<p>A) BEAS-2B cells were seeded in a 12-well tissue culture plate and a 0.4 uM pore size transwell i...