Inhibitors of PI3K increased chromosome lagging and prolonged the duration of prometaphase.

<p>HeLa cells were treated with 5 mM 3-MA or 50 µM wortmannin, and live cell imaging was performed for 48 hours. (A) Representative live cell imaging records showing a cell that stayed at prometaphase for more than eight hours (top panel), with the presence of lagging chromosomes outside the metaphase plate (bottom panel). (B) Quantification of 3-MA- or wortmannin-induced lagging chromosomes at prometaphase. n: the number of total cells. *: p<0.001, 2×2 χ² test, compared to the control. (C) Quantification of the duration of prometaphase under 3-MA or wortmannin treatment. n: the number of total cells. N: the number of cells that died in mitosis. *: p<0.001, student's t-test, compared to the control. Error bars represent standard deviations from the results of two independent experiments. Time is shown as hours: minutes.