PR binding associated with progestin responsive genes.

<p>Gene expression profiles in T-47D and AB32 cells were determined at 2, 6 and 24 h after treatment with 10 nM ORG2058. Transcripts that were significantly differentially expressed at 2, 6 and/or 24 h, compared to untreated controls, were considered progestin regulated and were compared with the list of PR binding regions in the same cell line. (A and B) Overlap between PR binding regions and progestin regulated genes in (A) T-47D and (B) AB32 cells. (C and D) PR binding regions within 10 kb or in the 5′-UTR of genes regulated at 2, 6 and 24 h in (C) T-47D and (D) AB32 cells. (E) Genes with patterns of progestin regulation in T-47D cells that grouped together were identified by SOM cluster analysis using Gene Pattern. Patterns of regulation are plotted as the mean log fold change relative to the untreated control. Error bars represent the standard error of the mean. (F) Mean distance to TSS of PR binding regions associated with each SOM cluster shown in (E).