Analysis of <i>ABI3</i>, <i>ABI4</i> and <i>ABI5</i> expression during the germination of wild type control and <i>hrs1-1</i> seeds.

<p>Quantitative PCR was conducted with gene specific oligonucleotide primers. The amplification of <i>ACT8</i> (<i>At1g49240</i>, encoding <i>Arabidopsis</i> ACTIN8) served as the internal control. The normalized expression levels (means ± SD) were each calculated using the results from three technical repeats, and are representative of three independent experiments. (A–C) Comparisons of <i>ABI3</i>, <i>ABI4</i> and <i>ABI5</i> expression levels between wild type (WT) control and <i>hrs1-1</i> samples collected at the indicated time points during germination on 1/2 MS medium or the media supplemented with 1 µM ABA or 100 mM NaCl. Asterisk indicates statistical significance from WT control at <i>P</i>≤0.05.