Analysis of plasmalemma H⁺-ATPase activity during the germination of wild type control, <i>hrs1-1</i> and complementation line.

<p>The seeds of wild type (WT) control, <i>hrs1-1</i> and complementation line CP6-13 were cold stratified for 48 h, followed by germination on 1/2 MS medium at 23°C. The levels of H⁺-ATPase activity (means ± SD, each calculated with the results of triplicate samples) were determined using plasma membrane enrich fractions prepared from the seed samples taken at the indicated time points. The data shown are typical of three independent experiments. The results obtained with the additional CP lines (CP19-1 and CP23-3) were highly similar to those determined for CP6-13 during the experiments. Single and double asterisks indicate statistical significance from WT control at <i>P</i>≤0.05 and 0.01, respectively.