Architecture and characteristics of φB124-14 genome.

<p><b>A.</b> Physical map of ΦB124-14 genome. <b>Outer track:</b> Position and orientation of each predicted ORF. Block arrows represent individual ORFs and indicate direction of transcription. ORF colour indicates functional assignment based on BlastP and conserved domain searches (minimum 20% identity, and an e-value of 1e⁻⁵ or lower), as well as analysis of the phage proteome, as described in the figure legend. ORFs marked with purple triangles indicate ORF function has been confirmed through LC-MS/MS analysis of the mature phage proteome (See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035053#pone-0035053-g004" target="_blank">Figure 4</a>). <b>Middle track:</b> Bars represent location of proposed functional gene clusters and ORFs belonging to each cluster. Colours of bars indicate putative role of each gene cluster in phage replication, based on predicted functions of member ORFs. <b>Inner track:</b> G+C content: dark grey lines = above average genome G+C content; light grey lines = below average genome G+C content. <b>B.</b> Percentage of ORFs assigned to each functional category, including unassigned ORFs. ORFs of unknown function are further broken down in a secondary pie chart to illustrate those with homologues in the other available <i>Bacteroides</i> phage genome (φB40-8), and those with no significant homology (nsh) to any sequences present in public databases encompassed by the nr dataset. <b>C.</b> Percentage of φB124-14 ORFs with highest homology (based on top hits by bit score in BlastP searches) to sequences of various phylogenetic origin. Only hits generating e values of 1e⁻⁵ or lower were considered significant in this analysis. nsh – no significant homology. <b>D.</b> Illustrates the percentage of predicted ORFs assigned to each of the three predicted functional modules in the φB124-14 genome.