Differences in colony morphology and percentages of infection during in vitro replication in cell lines.

<p>Giemsa stained preparations show the colony morphology of <i>A. marginale</i> VA in (A) Vero cells, (B) DAE100T cells, (C) BME26 cells, (D) ISE6 cells, (E) RF/6A cells, and (F) IDE12 cells. Arrows point to the <i>A. marginale</i> VA colonies growing in each cell line. Cell nuclei stained in lighter red-purple color and marked with the letter n. Bar = 20 µm. (G) Increase in the percent of infected tick and mammalian cells during a 12-day period. The solid red line with red circles represent the infection rate of ISE6 cells at each time point. The solid blue line and blue circles represent IDE12. Solid black line and triangles represent to the infection rates in BME26 cells, whereas the green squares and line represent to the values for the infection rates in Vero cells. The linear regression calculated from the percent infected cells over time is shown as a dotted line in the color corresponding to the cell line represented.Bars represent the standard deviation for each time point.