Alterations in insulin signaling impact female CHC profiles.

<p>Using both GC/MS and LDI-MS analysis, we found dramatic differences in female CHC profiles caused by mutation of the insulin-receptor substrate <i>chico</i>, which results in a reduction in insulin signaling <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002684#pgen.1002684-Clancy1" target="_blank">[27]</a>. Data points representing age-dependent measures of the same compound are connected by lines; <i>chico</i> mutant data are plotted in yellow and those of control in green. Ages of the flies at the time of measurement (6, 23, 37, and 48 days) are further distinguished by shading. Error bars are omitted for clarity. A few compounds are identified by both methods: C₂₅H₄₈ (LDI-MS) corresponds to 9,13-C25:2 and 7,11-PD (GC/MS); C₂₇H₅₂ to 7,11-HD and 5,9-HD; and C₂₉H₅₆ to 7,11-ND. Significant changes with aging are not indicated but are similar to published data <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002684#pgen.1002684-Kuo1" target="_blank">[36]</a>. P-values reflect differences in individual compounds between <i>chico</i> and control flies determined by the genotype main effect in a two-factor ANOVA. *P<0.05, **P<0.01, ***P<0.001.