Locations of <i>acuI</i> relative to various <i>ddd</i> and <i>dmdA</i> genes in different bacteria.

<p>Locations of the <i>acuI</i> genes relative to those that encode DMSP demethylase (<i>dmdA</i> – yellow fill) or the <i>ddd</i> genes that encode the DddD, DddL, DddP and DddY DMSP lyases (arrows filled with various colors) are shown. Grey-filled arrows signify genes with other, known roles in DMSP catabolism. The <i>fccA</i> and <i>fccB</i> genes in <i>Shewanella</i> sp. MR4 encode a flavocytochrome <i>c</i> and a tetraheme cytochrome <i>c</i> respectively. Gene tags from left to right as shown above are: <i>Halomonas</i> HTNK1: ACV84065 to ACV84073 inclusive <i>Rhodobacter sphaeroides</i> 2.4.1: RSP_1433 to RSP_1435 inclusive <i>Canididatus</i> Puniceispirillum marinum IMCC1322: SAR116_1428, SAR116_1427 <i>Alcaligenes faecalis</i> M3A: ADT64689 to ADT64696 inclusive <i>Arcobacter nitrofigilis</i> DSM 7299: Arnit_0113, Arnit_0112 <i>Shewanella</i> sp. MR4: Shewmr4_2154 to Shewmr4_2151 inclusive <i>Ruegeria pomeroyi</i> DSS-3: SPO1913, SPO1914 Also shown are the dimensions of the <i>Ruegeria pomeroyi</i> DSS-3 <i>dmdA-lacZ</i> and <i>acuI-lacZ</i> fusion plasmids (pBIO2020 and pBIO2021 respectively) in which the reporter <i>lacZ</i> gene in pBIO1878. The cloned <i>R. pomeroyi</i> DNA (shown as blue lines) was cloned into pBIO1878 to form pBIO2022 (<i>acuI+dmdA</i>) and pBIO2024 (<i>dmdA</i> alone) for the complementation tests.