Increased IFN-γ production and hepatic transaminases activity in <i>T. cruzi</i> infected WT and IL-17RA KO mice after neutrophil depletion.

<p>A) CD11b+Gr-1+ frequency and absolute numbers in blood, spleen and liver of 20-day <i>T. cruzi</i> infected WT and IL-17RA KO mice treated with anti-Ly6G mAbs. Data are shown as mean ± SD, n = 6–8 mice per group. P values calculated by two-tailed T test. B) Concentration of IL-10 determined in 48 h unstimulated culture supernatants of spleen and liver cell suspensions obtained from 20-day <i>T. cruzi</i> infected WT and IL-17RA KO mice treated with anti-Ly-6G mAbs. Each symbol represents a different mouse and horizontal line indicates the mean. P values calculated with two-tailed T test. C) Plasma IFN-γ and TNF concentration in 20-day <i>T. cruzi</i> infected WT and IL-17RA KO mice treated with anti-Ly-6G mAbs. Each symbol represents a different mouse and horizontal line indicates the mean. P values calculated with two-tailed T test. D) Activity of ALT and AST determined in the plasma of 20-day <i>T. cruzi</i> infected WT and IL-17RA KO mice treated with anti-Ly-6G. Data are shown as mean ± SD, n = 6–8 mice per group. P values calculated using two-tailed T test. E) Parasitemia determined in 20-day <i>T. cruzi</i> infected WT and IL-17RA KO mice treated with anti-Ly-6G Abs. Data are shown as mean ± SD, n = 6–8 mice per group. P values calculated using two-tailed T test. F) Survival of <i>T. cruzi</i> infected WT and IL-17RA KO mice treated with anti-Ly-6G. P value calculated with a Gehan-Breslow-Wilcoxon test, n = 12 per group. Data in A–D and in E are representative of three and two independent experiments, respectively.