EZH2 knockdown significantly increases mRNA levels of CIITApIV and <i>HLA-DRA</i> but not CIITApIII in MDA-MB 435 variants.

<p>(A–B) MDA MB 435 variants were transfected with either EZH2 specific siRNA or with control siRNA and were stimulated as indicated with IFN-γ. Levels of <i>HLA-DRA</i> mRNA and CIITApIV mRNA (A) and levels of CIITApIII mRNA (B) were measured by Q-PCR and were normalized to levels of GAPDH mRNA. Q-PCR was performed in triplicate and results shown represent the mean ± SD of three independent experiments. ***P<0.0005, **P<0.005, *P<0.05.