Increased lability of nucleosomes at chromatin entry sites correlates with MSL binding.

<p>Average enrichment profiles of chromatin properties around MREs are shown for S2 and Kc cells. Nucleosome density and successive salt extracted fractions of MNase-treated chromatin from S2 and Kc cells were described previously <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002646#pgen.1002646-Stenberg1" target="_blank">[28]</a>. Profiled chromatin properties comprise nucleosome density (black) and successive salt extracted fractions of MNase-treated chromatin: low salt (pink, 80 mM NaCl), high salt (blue, 600 mM NaCl), and pellet (green, salt insoluble). The dashed red lines on each plot indicate the MRE centers. In male S2 cells, nucleosomes are more labile at the MREs on the X chromosome in general, and nucleosome density is especially low at functional MREs. The relative lability of nucleosomes on the X chromosome is not seen in female Kc cells, and the extent of nucleosome depletion at functional MREs is less pronounced. See also <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002646#pgen.1002646.s004" target="_blank">Figure S4</a>.