Assembly of contigs and gap closure.

<p>(A) Five contigs were assembled de novo using 159,077 sequence reads generated by pyrosequencing, providing an estimated coverage of 60X with 5 gaps. (B) The gaps were filled by PCR and Sanger sequencing. Blue arrows indicate positions of primers used for PCR. Gaps 2 and 5 contained direct repeats (DRs) necessitating synthesis and sequencing of additional internal PCR fragments. DRI contains a 69 bp sequence repeated 2.3X; DRII contained an 85 bp sequence repeated 10.4X; and DRIII contained a 25 bp sequence repeated 7.0X. The non-repetitive I (NRI) and NRII sequences flank DRI. ORFs are indicated by numbered yellow arrows.