Sea buckthorn sequences associated with isoprenoid biosynthesis by A) cytosolic MVA pathway and B) plastidial MEP pathway.

<p>Enzymes for which sea buckthorn sequences have been identified are shown in green. Abbreviations used for intermediates are: 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA); mevalonic acid (MVA); mevalonate-5-phosphate (MVAP); MVAPP mevalonate-5-diphosphate (MVAPP); Isopentenyl diphosphate (IPP); Dimethylallyl-diphosphate (DMAPP); d-glyceraldehyde-3-phosphate (GAP); 1-deoxy-D-xylulose-5-phosphate (DXP); 2- C-methyl-D- erythritol-4- phosphate (MEP); 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol (CDP-ME); 2-phospho-4-(cytidine 5'C-diphospho) 2-C-methyl-D-erythritol (CDP-ME2P); 2-C- methyl-D- erythritol 2,4-cyclodiphosphate (MECDP); 1-hydroxy-3-methyl-2-(E)-butenyl-4-diphosphate (HMBPP); Abbreviations used for enzymes are: Acetoacetyl-CoA thiolase (AACT); 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS); HMG-CoA reductase (HMGR); mevalonate kinase (MVK); diphosphomevalonate kinase (PMK); diphosphomevalonate decarboxylase (PMD); isopentenyl diphosphate isomerase (IPP/IPI); 1-deoxy-d-xylulose-5-phosphate synthase (DXS); 1-deoxy-d-xylulose-5-phosphate reductoisomerase (DXR); 4-diphosphocytidyl-2-C-methyl-d-erythritol synthase (ISPD/CMS); 4-(cytidine 5′-diphospho-2-C-methyl-d-erythritol kinase (ISPE/CMK); 2-C-methyl-d-erythritol 2,4-cyclodiphosphate synthase (ISPF/MCS); 4-hydroxy-3-methyl but-2-enyl diphosphate synthase (ISPG/HDS); 4-hydroxy-3-methyl but-2-enyl diphosphate reductase (ISPH/HDR).