The effect of TIMP-1-GPI on t-PA, PAI-1, TNF-α and uPA protein secretion on human mesothelial cells.

<p>A quantitative assessment of protein expression was analyzed using specific ELISA and activity assays after 12 h TIMP-1-GPI treatment. (<b>A</b>) t-PA release was increased in dose dependent manner of TIMP-1-GPI whereas the secretion of PAI-1 (<b>B</b>) decreased under the same conditions. (<b>C</b>) The release of uPA was decreased in human mesothelial cells when the cells after 12 h treatment with TIMP-1-GPI. (<b>D</b>) TNF-α protein was also reduced by treatment with TIMP-1-GPI. Denatured dTIMP-GPI had no effect.