CDR-H3 physicochemical properties imply a normal antibody repertoire in humanized mice devoid of autoimmune signatures.

<p>(A) The average hydropathic index of the CDR-H3 interval was calculated using a normalized Kyte-Doolittle scale <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035497#pone.0035497-Eisenberg1" target="_blank">[38]</a>. The average hydropathy for all samples was centered about the neutral, slightly hydrophilic zero value and all were depleted of either highly charged (≤–0.7) or highly hydrophobic (≥0.7) CDR-H3s. (B) Pie charts showing the statistically similar proportion CDR-H3s with ≥0,1,2,3, or 4 positive charges, namely, the cationic residues arginine (R, <i>top row</i>) and lysine (K, <i>bottom row</i>). (C) Similarity of individual a.a. distributions within CDR-H3.