Gb3 impairs eNOS expression in HMiVECs.

<p><b>A.</b> Gb3 inhibits total eNOS expression in TNF-α stimulated HMiVECs and under basal conditions. Values are indicated as percent of unstimulated control. *p<0.05 vs unstimulated control; **p<0.05 vs TNF-α alone. <b>B.</b> Gb3 inhibits Ser₁₁₇₇ pho eNOS expression in TNF-α stimulated HMiVECs and under basal conditions. Values are indicated as percent of unstimulated control. *p<0.05 vs unstimulated control; **p<0.05 vs TNF-α alone. Values are representative of at least 4 different experiments; all blots are normalized to GAPDH expression. <b>C./D.</b> In all cases expression remained unchanged following GLA gene silencing.