Hippocampal NSCs can be isolated and established as a two-dimensional culture system.

<p>(a–c) Undifferentiated hippocampal NPCs are undifferentiated, expressing insignificant levels of Tuj1 and Doublecortin (DCX). (d–f) Three days following initiation of differentiation, individual progenitor cells become Tuj1 (+) and begin to strongly express DCX. (g, h) Neurogenesis proceeds from focal clusters which expand and contact one another within 7 days of induction of terminal differentiation. (i) Camera lucida of representative of expanding clusters of Tuj1(+) neural progenitors at 12, 24, 48 and 72 hours following differentiation.