The effect of culturing before inoculation of <i>Wolbachia</i> on ovary density.

<p>Ovaries were inoculated with <i>Wolbachia</i> immediately after culture (day 0) or 6 days post culture for <i>An. gambiae</i> (Ag), <i>An. stephensi</i> (As), <i>C. tarsalis</i> (Ct), <i>Ae. aegypti</i> (Aa) and <i>D. melanogaster</i> (Dm). Two days post-infection, the density of <i>Wolbachia</i> was compared in these two treatments. Density of <i>Wolbachia</i> in ovaries was significantly different (P<0.007) between the <i>Ae. aegypti</i> day 0 and day 6 treatments. For each insect species, five pools, each containing five pairs of ovaries, were evaluated for <i>Wolbachia</i> density. Error bars indicate SEM.