Generation of Reactive Oxygen Species (ROS) by phagocytic RAW264.7 cells upon infection with <i>M. genitalium</i> strains.

<p><b>A. Confocal images of RAW264.7 cells showing ROS generation.</b> RAW264.7 cells were infected with G37, MS5, MGRE or treated with heat killed G37 (MOI 1∶10) and generation of ROS was detected by addition of DCF-DA. Images were captured using an Olympus confocal laser scanning microscope with 488 nm laser. DCF-DA and DIC indicate fluorescence of DCF-DA and differential interference contrast of the same field; G37, MS5 and MGRE represent cells infected with <i>M. genitalium</i> wild type, <i>msrA</i> mutant and control strains. HKG37 represents cells treated with heat killed wild type <i>M. genitalium</i>. <i>t</i>-BHP represents ROS induced with 1 µM <i>t</i>-butyl hydroperoxide for 30 min. <b>B. Graphical representation of ROS generated by RAW264.7 cells.</b> Each bar represents ROS generated by RAW264.7 cells in response to infection/induction. Images were captured using a confocal laser scanning microscope. Total fluorescence counts were determined from images using NIH image J software from ten different fields and three independent experiments. Arbitrary fluorescence units for each infection are given as Mean ± SD. * = p≤0.05 vs wild type G37. Labels are as described in “<b>A</b>”.