FLJ20420 mRNA expression in different tissues and cell lines.

<p>Northern blot analysis of FLJ20420 mRNA expression in human normal tissues (A) and various tumor cell lines (B). FLJ20420 cDNA was used as a probe. The number on the left side indicates the size of the molecular mass markers. The same blot was then stripped and re-probed with human actin probe as an internal control. (C-D) Real-time PCR analysis of FLJ20420 and BAG-1 mRNA expression in lung cancer cell lines. NL9980 cells were used as a calibrator. The expression of FLJ20420 opposed BAG-1 expression in these cell lines. Error bars are representative of an average of 2 triplicate RT-PCR experiments. (E) Immunoblot analysis shows that BAG-1 protein expression corresponds to BAG-1 mRNA expression in the cell lines analyzed.