Knockout of IFN-γ suppresses Ang II infusion-induced inflammatory cells infiltration and cytokines expression in hearts.

<p><b>A–C</b>, Flow cytometry analysis of (<b>A</b>) CD45⁺, (<b>B</b>) CD3e⁺, (<b>C</b>) F4/80⁺ cells infiltration in Ang II-treated WT and IFN-γ-KO hearts. Bar graph shows quantification of different inflammatory cells in hearts (<i>n</i> = 5). <b>D</b>, Immunohistochemistry of expression of Mac-2 in WT and IFN-γ-KO hearts with saline or Ang II treatment: hematoxylin and eosin (HE) staining (<i>top panel</i>) and antibodies against Mac-2 (<i>bottom panel</i>). Bar graph shows quantification of areas of Mac-2⁺ cells. Magnification: ×200. <b>E–F</b>, Real-time PCR analysis of (<b>E</b>) tumor necrosis factor (TNF)-α Mrna and (<b>F</b>) macrophage inflammatory protein (MIP)-1α expression in both WT and IFN-γ KO mice at baseline and day 3, day 7 after Ang II infusion. Data are mean ± SEM (<i>n</i> = 5). * <i>p</i><0.05, ** <i>p</i><0.01 versus saline control; # <i>p</i><0.05 versus Ang II-infused WT mice.