IFN-γ deficiency decreases MCP-1 expression in Ang II-treated mouse hearts.

<p><b>A,</b> Immunoflourescence staning for α-SMA (red), MCP-1 (green) and DAPI (blue) in the Ang-II-infused hearts of WT mice. Arrowhead indicate α-SMA<b>^–</b>/MCP-1⁺ cells. <b>B,</b> The cryosections of heart were analyzed by double-immunofluorescence staining with antibodies against MCP-1 (green) and F4/80 (red, monocytes/macrophages). DAPI (blue) indicates nuclear staining. Magnification: ×400. **<i>p</i><0.01 versus saline control; # <i>p</i><0.05 versus Ang II-infused WT mice. <b>C,</b> MCP-1 protein level (by CBA) in WT and IFN-γ-KO hearts treated with saline or Ang II for 7 days (<i>n</i> = 5). <b>D,</b> Real-time PCR quantitative analysis of MCP-1 mRNA expression (<i>n</i> = 5).