Crosstalk between IFN-γ-producing T cells and macrophages augments IFN-γ and MCP-1 expression and macrophage migration.

<p><b>A</b>, IFN-γ expression was determined by incubating T cells either with macrophages isolated from and WT or IFN-γ-KO mice or with the supernatant of macrophages. Bar graph represents the quantification of IFN-γ level in the supernatant of the cell culture. <b>B</b>, Transwell assay of macrophages migration. WT macrophages were plated on the upper chambers of transwell inserts. The lower chamber was consisted of co-cultured WT or IFN-γ-KO T cells with WT macrophage (as described in <b>A</b>, <i>upper panel</i>). Macrophages that had migrated to the lower chamber were counted by DAPI staining. Bar graph represents the cell numbers that migrated into the lower chamber. <b>C–D</b>, Quantification of (C) MCP-1 or (D) CCL5 levels in the supernatant of the cell cultures as described in <b>A</b>. * <i>p</i><0.05, **<i>p</i><0.01 versus co-culture of IFN-γ-KO T cells with WT macrophages. Mφ, macrophage.