<p>The top line indicates the full-length CmRyR cDNA in kilobases. Thin lines (P1–P14) represent CmRyR cDNA clones obtained by RT-PCR. Solid lines (R5 and R3) represent CmRyR cDNA clones obtained by RACE.</p
<p><b>A. </b><i>B. subtilis</i> Gateway expression vector pDG148-GW with an inducible P<i>spac</i> p...
<p>(A) PCR amplification products from each of six multiplex PCR (A, B, C, O, H, and H3) separated b...
<p>The amplified product should contain the ATG. The blunt-end DNA segment can be cloned in all the ...
<p>The thick black line indicates the full-length <i>Of</i>RyR cDNA. S1–S17 represent the nucleotide...
Promoter entry clones are constructed by a BP reaction between pDONR P4-P1R and an attB4-promoter-at...
<p>The upper part of the figure shows the positions of the 5′ ends of plus (pos. 2489) and minus str...
<p>The arrows indicate the primers' annealing positions. The dotted line shows the sequence obtained...
<p>Solid lines represent RNA genomes and boxes represent ORFs. Putative protein products are indicat...
<p>The sequences of the primers used for RT-PCR, DNA pull-down assays and chromatin immunoprecipitat...
<p>The black, blue and red line separately represents the total number of the <i>R</i> genes, <i>R</...
Verification of the cpnC- mutant cell line using PCR and genome sequencing. A) Diagram indicating wh...
<p>Note: R = A/G, Y = C/T, M = A/C, K = G/T, S = C/G, W = A/T, H = A/C/T, B = C/G/T, V = A/C/G, D = ...
Promoter entry clones are constructed by a BP reaction between pDONR P4-P1R and an attB4-promoter-at...
<p>Each row represents an individual sequence clone, grouped together for each allele size, from cul...
The principal of cdna strand was that it was combined from complete rna utilizing an oligo(dt)- cont...
<p><b>A. </b><i>B. subtilis</i> Gateway expression vector pDG148-GW with an inducible P<i>spac</i> p...
<p>(A) PCR amplification products from each of six multiplex PCR (A, B, C, O, H, and H3) separated b...
<p>The amplified product should contain the ATG. The blunt-end DNA segment can be cloned in all the ...
<p>The thick black line indicates the full-length <i>Of</i>RyR cDNA. S1–S17 represent the nucleotide...
Promoter entry clones are constructed by a BP reaction between pDONR P4-P1R and an attB4-promoter-at...
<p>The upper part of the figure shows the positions of the 5′ ends of plus (pos. 2489) and minus str...
<p>The arrows indicate the primers' annealing positions. The dotted line shows the sequence obtained...
<p>Solid lines represent RNA genomes and boxes represent ORFs. Putative protein products are indicat...
<p>The sequences of the primers used for RT-PCR, DNA pull-down assays and chromatin immunoprecipitat...
<p>The black, blue and red line separately represents the total number of the <i>R</i> genes, <i>R</...
Verification of the cpnC- mutant cell line using PCR and genome sequencing. A) Diagram indicating wh...
<p>Note: R = A/G, Y = C/T, M = A/C, K = G/T, S = C/G, W = A/T, H = A/C/T, B = C/G/T, V = A/C/G, D = ...
Promoter entry clones are constructed by a BP reaction between pDONR P4-P1R and an attB4-promoter-at...
<p>Each row represents an individual sequence clone, grouped together for each allele size, from cul...
The principal of cdna strand was that it was combined from complete rna utilizing an oligo(dt)- cont...
<p><b>A. </b><i>B. subtilis</i> Gateway expression vector pDG148-GW with an inducible P<i>spac</i> p...
<p>(A) PCR amplification products from each of six multiplex PCR (A, B, C, O, H, and H3) separated b...
<p>The amplified product should contain the ATG. The blunt-end DNA segment can be cloned in all the ...
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