NCoR and CtBP1 act as functional co-repressors of Snail2 depending on the SNAG and the SLUG domains.

<p>The co-repressor action of NCoR (A), mSin3A (B) and CtBP1 (C) in the Snail2-mediated repression on the mouse <i>E-cadherin</i> promoter was analyzed on HEK293T cells. The <i>E-cadherin</i> promoter activity was determined in the presence of 50 ng of Snail2-HA wild type, the ΔSNAG or the ΔSLUG mutants and, when indicated, in the presence of 50 ng of NCoR, mSin3A, or CtBP1. Reporter assays were performed as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036132#s4" target="_blank">Material and Methods</a>, and relative luciferase units (RLU) normalized to the activity obtained in the presence of a void control pcDNA3-HA vector. Results show the mean of at least triplicate experiments, performed on quadruplicate samples, +/− s.d. *p<0.05; **p<0.005; ***p<0.001; n.s, not significant.