Overexpression of <i>HsfA2</i> leads to constitutive expression of its target genes in <i>rps1</i> mutant plants.

<p>(A) qRT-PCR analysis of mRNA levels of the representative <i>HsfA2</i> target genes in the fully-extended leaves of WT, <i>rps1</i> and <i>rps1</i> with a <i>35S:HsfA2</i> cDNA transgene plants. <i>Actin2</i> was used as the internal standard. Error bars indicate standard deviations of three technical replicates, and the results were consistent in three biological replicates. (B) Western blot analysis of thylakoid membrane proteins extracted from the leaves of the genotypes indicated in (A). Equal protein loading was determined by contents (2 µg) of chlorophyll in thylakoid membrane extracts according to (Peng et al., 2006). (C) Protein levels of HSP21, encoded by <i>HsfA2</i> target gene <i>Hsp25.3</i>, in leaves of the plants described in (A) were analyzed by western blots with a polyclonal antibody against HSP21.