Innate mouse multipotent mesenchymal C3H10T1/2 cells expressed multiple lineage-specific marker genes.
- Kayo Suzuki (166737)
- Yoshito Matsui (166741)
- Mami Higashimoto (166744)
- Yoshiharu Kawaguchi (166748)
- Shoji Seki (166752)
- Hiraku Motomura (166756)
- Takeshi Hori (166759)
- Yasuhito Yahara (166761)
- Masahiko Kanamori (166764)
- Tomoatsu Kimura (166767)
Publication date
May 2012
DOIAbstract
<p>RT-PCR analysis detected osteoblastic marker Opn (lane 1), chondrocytic marker Col11a2 (lane 3), and adipocytic marker Ppar-γ (lane 5) mRNA transcripts in C3H10T1/2 cells. Mouse osteosarcoma cell line LM8 (lane 2), mouse embryonic skeleton cells (lane 4), and mouse preadipocytic cell line 3T3-L1 (lane 6) were analyzed as positive controls for Opn, Col11a2, and Ppar-γ gene expression, respectively. The β-actin transcript level served as a loading control for each reaction.</p
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