Functions of the SET-16/UTX-1 complex.

<p>(A) (Upper panel) DIC images of <i>set-16(n4526)</i> and <i>pis-1(ok3720)</i> mutant larvae. Arrowhead indicates misshapen tails. Scale bar is 50 µm. (Lower panel) DIC images of <i>set-16(RNAi)</i> and <i>pis-1(ok3720)</i> adults. Gonadal migration defects are shown. The black line indicates the aberrant gonadal migration. Scale bar is 20 µm. Animals are oriented anterior to the left. The percentage of tail and gonadal defects associated to loss or reduction of <i>set-16</i> and <i>pis-1</i> are reported on the right. (B) Percentage of tail and gonad defects after <i>RNAi</i> of the indicated genes. F1 L1 larvae and adult animals from at least two independent experiments were scored. (C) Percentage of tail and gonad defects after <i>RNAi</i> of the indicated genes. F1 or F2 L1 larvae and adult animals from at least two independent experiments were scored. (D) <i>utx-1</i> and <i>set-16</i> mRNA levels in embryos of worms treated with control, <i>utx-1</i> or <i>set-16(RNAi)</i>. *P<0.01 (Student's t-test). (E) Protein lysates of embryos from worms treated with control, <i>utx-1</i> or <i>set-16(RNAi</i>) were probed with an antibody against UTX-1. Actin was used as loading control. The signal was quantified using ImageJ program and normalized to actin. Indicated values are relative to <i>control (RNAi)</i> and derive from two independent experiments.