P-element insertion point detection:

<p>Schematic figure showing P-element transposon P(BmΔ-w) insertion site. Sequencing results shows P-element insertion site (arrow) within the genomic DNA of <i>Btk29A</i> at 2L: 8277721. GTCGAGCGC repeats can be seen at both end of the P insertion, which is a characteristic mark of transposon insertion. This information was further use to distinguish between mutant and revertant flies. In mutant flies this breakpoint was easily detected in amplicons (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035640#s3" target="_blank">Materials and Methods</a>), whereas the revertant did not show any P insertion.