Global patterns of gene expression differences among sample groups:

<p><i>A</i>) <b><i>Hierarchical clustering:</i></b> Neighbor joining tree based on pairwise distance matrices (1−ρ, Spearman correlation coefficient) for the different sample groups within this study. Sample groups fall out based on the strongest factor, larval CNS or adult head, respectively, i.e. developmental stage. Thereafter on either <i>Btk29A</i>^ficP (red) or revertant (<i>Btk29A</i>^{(fic Exc1–16)}) (green). <b><i>B</i></b><i>) </i><b><i>Factorial map of the principal-component analysis:</i></b> The replicates within each sample collection are grouped together based on the individual samples whole genome expression profile. Revertant is denoted with Δ and ficP with a o. All samples are colored individually. The proportion of the variance explained by the principal components (axis) is indicated in parentheses in the graph. <b><i>C</i></b><i>) </i><b><i>Sample correlation matrix:</i></b> Spearman correlation (R²) is calculated and visualized by color (red-blue) in the matrix. Within the replicates for the individual sample groups the correlation is higher than between the sample groups. Also, the correlation within developmental stage was found to be higher compared to between mutant strains. Sample names are color-coded with red (<i>Btk29A</i>^ficP) and green (revertant).