Changes in fatty acid composition during physic nut seed development.

<p>A: Gas chromatography of fatty acids in mature physic nut seeds. The peaks were identified by mass spectrometry. B: Fatty acid composition in mature physic nut seeds. Each data point represents the mean (±SD) of three biological replicates. C: Changes in fatty acid composition during seed development. Fatty acids were extracted from whole seeds sampled 14 and 19 days after pollination (DAP) and from kernels of seeds sampled 25 to 45 DAP. Each data point represents the mean (±SD) of three biological replicates. C17∶0 was used as both a seed lipid extraction and transmethylation internal standard. C16∶0, palmitic acid; C16∶1 palmitoleic acid; C18∶0, stearic acid; C18∶1, oleic acid; C18∶2, linoleic acid; C18∶3, linolenic acid; C20∶0, arachidic acid; C20∶1, gondoic acid; 12-OH C18∶1, 12-hydroxyoleic acid (ricinoleic acid).