<i>rrf-1</i> mutants have increased <i>sod-3</i> mRNA levels, a normal lifespan, and normal thermo-tolerance.

<p>(<b>A</b>) The <i>sod-3 </i>mRNA levels of a mixed population of <i>C. elegans</i> wild-type (WT, N2(A)) and <i>rrf-1(pk1417).4x</i> (<i>rrf-1(pk1417)</i> mutant outcrossed four times to WT(A)) strains were determined. Bars show the mean + SEM of three independent experiments; **P<0.005 (Student’s <i>t</i>-test)<b>.</b> (<b>B</b>) Lifespan analysis of wild-type WT(A) and <i>rrf-1(pk1417).4x</i> strains at 20°C; P = 0.59, log-rank (Mantel-Cox test). This experiment has been performed three times with similar results; see <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035428#pone.0035428.s007" target="_blank">Figure S7F</a></b> for additional data. (<b>C</b>) Thermo-tolerance was measured by assessing survival of WT(A) and <i>rrf-1(pk1417).4x</i> strains after 8 h incubation at 36°C; P = 0.36 (Student’s <i>t-</i>test). This experiment has been performed three times with similar results.